Identification of flavonols in polar extract in Aciotis polystachya (Bonpland) Triana (Melastomataceae)
DOI:
https://doi.org/10.18636/bioneotropical.v4i1.120Keywords:
Aciotis polystachya, Flavonols, Melastomataceae, Kaemphferol, Quercetin, NMR.Abstract
Objetive: To isolate, to identify and to elucidate the secondary metabolites present in the same fraction extract ethyl acetate of air parts of Aciotis polystachya (Bonplan) Triana (Melastomataceae). Methodology: Techniques using column chromatography, thin layer chromatography and preparative chromatography was isolate, identify and purify secondary metabolites, the analysis of the spectra of 1H-NMR, 13C-NMR and two-dimensional NMR techniques (1H-1H COSY and HMQC) allowed us to elucidate the chemical structure of these metabolites. Results: The methanol extract was performed Aciotis polystachya solid to liquid extraction with ethyl acetate, fractions 57-64 from the chromatography of the extract usually ethyl acetate, eluted with solvent dichloromethane-ethyl acetate in volumetric proportions 40-60%, 0.03 grams was isolated as a yellow solid after successive chromatographies, similarly fractions 65-73 and 57-64 from the same chromatography was obtained 0.13 grams of total product as yellow crystals. Conclusions: Quercetin and Kaempherol are new secondary metabolites report both gender as this species, and are responsible for many biological and pharmacological activities, which justify some ethnobotanical uses of this plant.
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