Clonal micropropagation and ex vitro rooting of of three cultivates of pineapple Ananas comosus (L. Merr.) from Chocó, Colombia
DOI:
https://doi.org/10.18636/bioneotropical.v4i2.199Keywords:
Cultivate, in vitro culture, Massive propagation, Micropropagation, Pineapple.Abstract
Objective: Massively propagate pineapple three cultivars of Chocó in Colombia, Ananas comosus (L. Merr.). Methodology: in vitro multiplied buds of apices extracted from crown and basal children cultured in semisolid Murashige and Skoog initiation medium (MS, 1962) containing: 4 mg. l-1 thiamine HCl, 0,5 mg/l NAA, 0,5 mg/l IBA and 0,5 mg/l 6-BAP and pH adjusted to 5,8; 10 g/l activated charcoal, and then a multiplication medium with four hormone treatments; (1) 0,3 mg/l of naphthalene acetic acid (NAA)+1,0 mg/l of benzylaminopurine (6-BAP), (2) 0,5 mg/L NAA+1,5 mg/l BAP, (3) 1,0 mg/l NAA+3,0 mg/l 6-BAP, (4) 1,0 mg/l NAA + 3,0 mg/l 6-BAP+2 mg/l of gibberellic acid (GA3). Rooting ex vitro was performed were planted in pots in a medium prepared with soil from ant, fine sand and moss in equal proportions (1:1:1), disinfested with formaldehyde a month before the process starts. Results: At 90 days, the number of shoots and shoots size of explants was: C1 (78,4±0,5) shoots and shoots length (2,5±0,03 cm); C2: (85,7±0,5) shoots and shoots length (2,1±0,11 cm), C3 (92,8±0,1) shoots and shoots length (1,6±0,07 cm). The best combination of growth regulators was 1 mg/l NAA and 3 mg/l de 6- BAP.lo indicating that the presence of auxin and citoquinia necessary for sprouting. The rooting was ex vitro, and at 90 days the plants had an average of: C1 (5,8±0,4) roots and root length (3,2±0,3 cm); C2 (5,4±0,1) roots and roots length (3,9±0,8 cm); C3 (5,7±0,7) roots and roots length (3,8±0,5 cm).
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References
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